Biology

 

Structural Biology and Modeling 中文 (CN version)


Structural Biology offers the highest quality gene to structure service with unparalleled expertise and capability. Our team has an excellent track record for successful completion of multiple projects with complex and unprecedented proteins.

Capabilities

  • Instruments
    • Clonpix for stable clone generation
    • Mosquito nano-liter robot
    • 7 AKTAs (including pilot, explorer, purifier and xpress)
    • TOF LC-MS
    • Wave bioreactors ( 5 L and 25 L)
    • 2.5 L/10 L Sartorius, 50 L SUB high clone
    • Access to synchrotron beam line in SSRF
  • Quality Control
    • SDS-PAGE/IEF
    • Western blotting or amino acid sequencing
    • HPLC
    • Mass spectroscopy, DLS, CD
    • Biochemical assay

Core Services

  • Expression systems
    • E. coli, insect cells, mammalian cells
  • Purification service
    • Combined methods of chromatography
    • Affinity, ion exchange, size exclusion, hydrophobic interaction etc . . .
    • Target to achieve protein purity >95% if necessary
    • Insoluble protein production and purification
  • Off-the-shelf crystallization service
    • hPARP1, GSK3b, hCLK2, BACE, HCV protease, NS5A, NS5B, HIV integrase, CaMKIIg, LXR/RXR, dehydrogenase, CYP 3A4
  • Structure service
    • Crystallization screening using Mosquito nano-liter robot
    • Data collection at Shanghai Synchrotron Radiation Facility (SSRF)
    • Determine crystal structure with MR, MIR, SIR, MAD and SAD methods
    • Model building with Coot, DS and Afitt
    • Structure refinement with CCP4 and CNS

CASE 1

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  • FBDD (Fragmentation-Based Drug Discovery)
    • WuXi fragment library with unique scaffolds
    • NMR screening with STD and WaterLOGSY
    • Obtain crystal of apo protein for FBDD
    • Crystal soaking of fragment cocktail
    • Design high affinity ligand based on weak binding fragment

CASE 2                                                                                        CASE 3

image083                    image085

 

Integration Service

  • From gene expression to structure determination to new ligand design

CASE 4

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